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Visitors:1197737
Primer tm:96161
Primer score:22329/14428
Search:17936/13158
Fast PCR:3277322/1315414
Primer design:106170/122175
MSP design:35418
Version:2.75
Last update:21/03/23

Calculate melting temperature of a primer

Primer sequence

Parameters

Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %

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Calculating melting temperature of a primer

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