Primer Design and Search Tool

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Primer search, ePCR

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Statistics

Visitors:

1197739

Primer tm:

96161

Primer score:

22329/14428

Search:

17936/13158

Fast PCR:

3277324/1315414

Primer design:

106170/122175

MSP design:

35418

Version:

2.75

Last update:

21/03/23

Search Methylated Specific Primers

Sequence
Bisulfite	Use sense or antisense chain.
Set search region for	Forward primer: - Reverse primer: -
Max length of PCR
Min tm diff
CpG sites	let only in one or both primers.

Parameters

Primer melting temperature

Primer conc	mikromol	Glycerol conc	%
Potassium conc	milimol	Ethylen glycol conc	%
Magnesium conc	milimol	Formamid conc	%

Primer scoring values

Description	Weight	Min	Opt	Max		Description	Weight	Max
Primer length						Self annealing
GC content						Self end-annealing
GC content (bis)						Pair annealing
Melting temp						Pair end-annealing

Primer design

Results list size

Max Tm diff

Minimum of CpGs

Help

Search the best primer pairs for PCR a sequence

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

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