BiSearch: Primer Design and Search Tool

Search Methylated Specific Primers

Bisulfite Use sense or antisense chain.
Set search region for Forward primer: - Reverse primer: -
Max length of PCR
Min tm diff
CpG sites let only in one or both primers.


Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %
Primer scoring values
Description Weight Min Opt Max    Description Weight Max
Primer length Self annealing
GC content Self end-annealing
GC content (bis) Pair annealing
Melting temp Pair end-annealing
Primer design
Results list size Max Tm diff Minimum of CpGs


Search the best primer pairs for PCR a sequence

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

Copyright © Institute of Enzymology :: Disclaimer :: Privacy