BiSearch: Primer Design and Search Tool

Menu

Primer tm

Primer score

Simple search

Primer search, ePCR

Primer design

MSP design

Parameters

Help

Manual

Faq

Manuscripts

ChangeLog

Genome builds

Comments

Statistics

Visitors:1197723
Primer tm:96161
Primer score:22329/14426
Search:17936/13158
Fast PCR:3277322/1315412
Primer design:106170/122168
MSP design:35418
Version:2.75
Last update:21/03/23

Calculate score of a primer pair

Forward primer
Reverse primer
Bisulfite

Parameters

Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %
Primer scoring values
Description Weight Min Opt Max    Description Weight Max
Primer length Self annealing
GC content Self end-annealing
GC content (bis) Pair annealing
Melting temp Pair end-annealing

Help

Calculating melting temperature of a primer

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

Copyright © Institute of Enzymology :: Disclaimer :: Privacy