BiSearch: Primer Design and Search Tool

Calculate score of a primer pair

Forward primer
Reverse primer


Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %
Primer scoring values
Description Weight Min Opt Max    Description Weight Max
Primer length Self annealing
GC content Self end-annealing
GC content (bis) Pair annealing
Melting temp Pair end-annealing


Calculating melting temperature of a primer

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

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