BiSearch: Primer Design and Search Tool

Search for the best primer pairs

Set search region for Forward primer: - Reverse primer: -
Max length of PCR


Primer melting temperature
Primer conc  mikromol Glycerol conc  %
Potassium conc  milimol Ethylen glycol conc  %
Magnesium conc  milimol Formamid conc  %
Primer scoring values
Description Weight Min Opt Max    Description Weight Max
Primer length Self annealing
GC content Self end-annealing
GC content (bis) Pair annealing
Melting temp Pair end-annealing
Primer design
Results list size Max Tm diff Minimum of CpGs
Database search and fast PCR
Database Mismatches  Max: 
PCR length PCR product to show
Primer matches to show


Search for the best primer pairs

Online help system

The dynamic Online help system informs you about each input field. Move the mouse over the input field you would like to fill in, and read the help here.

Copyright © Institute of Enzymology :: Disclaimer :: Privacy